Effects of Chlorophyll in Papaya Leaves on Superoxide Dismutation and Blood Glucose Level of Diabetic Rats
Abstract: Hyperglycemia in
diabetes mellitus results in oxidative stress and increases complication
development. Experimental studies have shown that chlorophyll has antioxidant
activity and papaya leaves contained chlorophyll more than the other green
vegetables. This study aimed to evaluate the antioxidant and hypoglycemic role
in chlorophyll rich in papaya leaves on diabetic rats. Thirty six rats were
randomly divided into 4 groups: without treatment (technique control/TC),
diabetic (negative control/NC), diabetic with treatment A (100.3 mg/200 g BW of
extract) and diabetic with treatment B (200.6 mg/200 g BW of extract). Diabetic
induction was conducted by injecting streptozotocin 40 mg/kg BW
intraperitoneally. Extract was given by nasogastric tube. Blood glucose level
was measured using enzymatic colorimetric GOD-PAP test at before, after 20 and
40 days of treatment. Hepatic superoxide dismutation (SOD) level was measured
after 40 days of treatment. Blood glucose levels in 3 diabetic groups were significantly
raised after seven days of induction. In Anova and post hoc LSD analysis, both
treatments had lower hepatic SOD level than TC (p=0.0001), and blood glucose
level also decreased after given the treatment (p=0.0001). Treatment B had a
better antioxidant and hypoglycemic role than treatment A.
Keywords: blood glucose;
chlorophyll; diabetes mellitus; papaya leaves; superoxide dismutation
Author: Choirun Nissa, Martha
Irene Kartasurya, Banundari Rahmawati
Journal Code: jpkedokterangg150379
