Destructive Effect of Calcium Hypochlorite against Pseudomonas aeruginosa Biofilm
Abstract: Pseudomonas
aeruginosa is the most common bacteria contaminating the hemodialysis water and
has high capability to form a biofilm. The presence of biofilm is hazardous
because it becomes a constant source of bacterial and toxin release toward the
hemodialysis patient’s blood. Calcium hypochlorite (Ca(OCl)2) is an easily
obtained disinfectant. This study was aimed to detect the destructive effect of
Ca(OCl)2 against P. aeruginosa biofilm and the optimal disinfectant concentration
required to achieve significant effect.
Methods: This experimental study was conducted in six replicates from
September to October 2015 in Microbiology Laboratory of Faculty of Medicine
Universitas Padjadjaran Bandung. A modified tissue culture plate method was
performed to grow P. aeruginosa biofilms which were subsequently treated with
Ca(OCl)2 in various chlorine concentrations, namely 20, 30, 40, and 500 parts
per million (ppm). The data was analyzed using Welch Analysis of Variance
(ANOVA) and Games-Howell post-hoc tests and presented in tables.
Results: Data were obtained from 36 flat-bottomed polystyrene wells.
There was a statistically significant mean difference between groups [F(4,
11.92)= 91.198, p<0.001)]. All of the tested chlorine concentrations caused
significant decreases in biofilm optical densities (p = 0.027 for 20 ppm and
p< 0.001 for 30, 40, and 500 ppm).
Conclusions: Ca(OCl)2 with chlorine concentrations of 20, 30, 40, and 500
ppm have significant destructive effect against P. aeruginosa biofilm. The mean
differences among treated groups were not significant. The most optimum
concentration is 30 ppm.
Keywords: Biofilm, calcium
hypochlorite, Pseudomonas aeruginosa
Author: Ilma Arifani, Gita
Widya Pradini, Insi Farisa Desy Arya, Adi Imam Cahyadi
Journal Code: jpkedokterangg170165
