Bryophyllum pinnatum leaves ethanol extract inhibit maturation and promote apoptosis of systemic lupus erythematosus BALB/c mice B cells
Abstract: B cells play a key
role in systemic lupus erythematosus (SLE). Targeting B cells as SLE therapy is
a plausible approach. This study investigated the potential effects of
Bryophyllum pinnatum leaves with ethanol extract in decreasing percentages of
maturation, increasing percentages of apoptosis, and decreasing NF-κB p65
expressions of SLE BALB/c mice B cells.
Methods: Culturing B cells from pristane induced SLE BALB/c mice’s spleen
will resulted in this in vitro study. B cells were activated by BAFF, LPS,
IL-4, and anti-CD40 yielding CD19+ >80%. B cells were cultured by adding
those stimulants with and without B. pinnatum leaves (0, 0.02, 0.1, or 0.5
µg/ml) for 72 hours at 37°C. Flow cytometry was performed to determine The
Percentages of maturation (CD19+CD38+) and apoptosis (Annexin V+PI+) of B
cells. Further analysis to determine the
expressions of transcription factor of maturation and apoptosis of B cells,
NF-ĸB p65, were performed using immunocytochemistry. Data were analyzed using
SPSS version 22.
Results: Flow cytometry assay showed significant decrease in percentages
of maturation of B cells in all doses and significant increase in percentage of
apoptosis of B cells in dose 0.5 µg/ml. Immunocytochemistry results showed
significant decrease expressions of NF-ĸB p65 in all doses. Percentages of
maturation, apoptosis, and expressions of NF-ĸB p65 of B cells were
significantly correlated.
Conclusion: This in vitro study revealed that B. pinnatum leaves with
ethanol extract decreased the percentages of maturation, increased the
percentage of apoptosis, and decreased NF-κB p65 expressions of SLE BALB/c mice
B cells significantly.
Keywords: B cells; Bryophyllum
pinnatum; pristane; SLE
Author: Kusworini Handono, Tri
W.I. Dantara, Elvira S. Dewi, Mirza Z. Pratama, Nurdiana Nurdiana
Journal Code: jpkedokterangg170232
