Analysis Expression of ZIP1 and Caspase-3 Protein in Adenocarsinoma of the Prostate
Abstract: Carcinogenesis of
adenocarcinoma of the prostate occurs due to dysregulation of zinc level within
the cells. Intracellular zinc molecules influx is regulated by a transporter
protein ZIP1, whose non-presence is predicted to inhibit apoptosis, thus leads
to the development of prostate adenocarcinoma. Methods: This study was aimed to
analyse the correlation of ZIP1 and Caspase-3 expression in prostate
adenocarcinoma on its grading as represented by Gleason Score. This was a
cross-sectional, retrospective analytical study on 31 Formalin-fixed,
paraffin-embedded tissue that meets inclusion criteria. The specimen was
stained using the immune-histochemistry technique for ZIP1 and Caspase-3.
Protein expression of each case was counted using ImageJ analysis. Gleason
score was acquired as secondary data from the cases' reports. The correlation
of their expression with respect to Gleason score was analysed with Pearson's
correlation using SPSS 11.5. Results: Mean expression level of ZIP1 and
Caspase-3 in prostate adenocarcinoma were 35% and 33%, respectively. There was
a significantly positive correlation between ZIP1 and Caspase-3 expression
(r=0.379; p=0.018). However, their correlation was stronger in
intermediate-grade group (r=0.73; p=0.01) and the correlation was much weaker
in high-grade group (r=0.04; p=0.48). Conclusions: There was a positive
correlation between ZIP1 and Caspase-3 expression in prostate adenocarcinoma.
Keywords: ZIP1; Caspase-3;
Gleason; apoptosis; adenocarcinoma; prostate
Author: Aditya D Septiawan,
Ria Kodariah, Meilania Saraswati
Journal Code: jpkedokterangg160325
