Addition of conjugated linoleic acid in whole milk improves lipid profile in high fat diet induced hypercholesterolemia of rats
Abstract: Conjugated linoleic
acid (CLA) is an isomer of linoleic acid that has been shown to have many
beneficial effects in prevention of atherosclerosis, hypertension,
cardiovasculardiseases and improve immune function. Although majority of CLA in
the diet are derivedfrom dairy product such as milk, however, the content of
CLA in milk is affected by cow’sdiet. The aim of this study was to investigate
the beneficial effect of CLA supplementation in milk for improving lipid
profile in high fat diet of rats. Twenty four male Sprague Dawley rats aged 8
weeks were given high fat diet for 3 weeks to induce hypercholesterolemia.Six
rats were maintained in standard diet as control. Rats then were divided into 4
groups i.e. normal control, negative control, high fat diet+CLA 0.5%, high fat
diet + CLA 0.5% supplemented skim milk, and high fat diet + CLA 0.5%
supplemented whole milk. Blood sample was drawn after high fat diet induced
hypercholesterolemia and after 4 weeks of treatment for total cholesterol,
triglyceride, low-density lipoprotein cholesterol (LDL cholesterol), and high
density lipoprotein cholesterol (HDL cholesterol) analysis. Bodyweight was
measured each week. Results showed that body weight was significantly increase
in all groups received high fat diet (p<0.05). There was no significant
differencein body weight between treatment group (p>0.05). Total
cholesterol, triglyceride, andLDL cholesterol was significantly decrease in
whole milk followed by significant increase in HDL cholesterol level. Skim milk
supplemented with CLA had only modest effect on triglyceride and HDL
cholesterol level. In conclusion, CLA supplementation in whole milkimproves
lipid profile in high fat diet.
Key words: conjugated linoleic
acid – hypercholesterolemia - lipid profle - milk - diet
Author: Arta Farmawati
Journal Code: jpkedokterangg160345
