THE GENETIC STRUCTURE OF THE WHITE CYPRINID (TOR TAMBROIDES) POPULATIONS BASEDON COIGENE SEQUENCE ANALYZES
Abstract: The study analyzed
nucleotide sequences from the mitochondrial cytochrome oxidase subunit (COI)
gene region (654) to investigate the genetic structure of the white cyprinid
(Tor tambroides) among nine populations from the Manna and Semanka Rivers. A
total of 36 individuals were collected for this work. Five nucleotides were
found to be variable, resulting in 4 haplotypes. Among the nine populations of
Melebuy in Semanka River represents the highest level of variability (h =
1.000, π = 0.0015) whereas Kerinjing, Merabung and Kotabumi populations
represent the highest level of variability in Manna River (h = 0.667, π =
0.0020). The Batu Aji, Air Sebilo and Kutopadang populations exhibit the lowest
level of variability (h = 0.000, π = 0.000). There is an integrated population
throughout all sample sites in Manna River. However, the AMOVA analysis
provided corroborating evidence for genetic structure obtained from Nei’s
genetic diversity statistic and the FST value (0.310), suggested there is
genetic divergence among populations of those populations. Of the total genetic
diversity, 35% was attributable to inter-population diversity and the remainder
(68.92%) to differences within populations. These two approaches produced a
picture of genetic structure in Manna and Semanka River. A molecular
phylogenetic tree constructed using The Neighbor-joining (NJ) method showed the
4 haplotypes were assigned to two clades associated geographic regions. These
results provide basic information for the conservation and sustainable
exploitation of this species.
Keywords: Tor tambroides; COI
genes; genetic structure; genetic variation; haplotype
Author: Arif Wibowo, Husnah
Journal Code: jpperikanangg120044
