Identification of SGS 1609 Cellulolytic Bacteria Isolated from Sargassum spec. and Characterization of The Cellulase Produced
Abstract: Bacterial isolate
from seaweed designated as SGS 1609 was previously found to be able to produce
cellulase represented by formation of clear zone on solid medium containing
carboxymethylcellulose (CMC). This research was conducted to identify the
isolate and determine optimum production time as well as characterize the
cellulase produced. The isolate was identified using 16s-rRNA gene analysis. Cellulase production
was conducted by cultivating the isolate in the liquid medium containing CMC
followed by centrifuging to get supernatant as the crude enzyme. The enzyme was
then concentrated using ammonium sulfate precipitation and ultra filtration.
The concentrated enzyme having higher activity produced from the concentration
process was then characterized to
determine its optimum pH and temperature, heat stabilization, metal ions effect
and substrate specificity. The result showed that the SGS 1609 isolate was identified
as Serratia marcescens with 99% similarity. The isolate produced cellulase
optimally at 4 days incubation. Ultra filtration produced higher enzyme
activity compared to NH4-sulfate precipitation. The enzyme concentrated by
ultra filtration worked optimally at the
pH of 7, temperature of 50 oC, stable at the temperature of 60 oC for
240 minutes and was increased its activity by Ca2+ and Mg2+ ions. On the other
hand, the enzyme was inhibited by Fe3+, Zn2+ and Na+ ions, but was not
relatively affected by K+ and EDTA. The use of conventional agar producer
waste treated with 6% NaOH gave highest
activity compared to other substrates.
Keywords: SGS 1609 bacterial
isolate, identification, cellulase, characterization
Author: Yusro Nuri Fawzya,
Stenny Putri, Nita Noriko, Gintung Patantis
Journal Code: jpperikanangg130020
