IDENTIFICATION OF MAJALAYA COMMON CARP STRAINS RESISTANT TO KHV INFECTION USING CYCA-DAB1*05 ALLELE AS THE MARKER
Abstract: The Cyca-DAB1*05
allele of major histocompatibility complex class II genes is recently suggested
to have a link with the European common carp strain resistant to koi
herpesvirus (KHV). In this study, a set of specific primers for Cyca-DAB1*05
was designed and applied as a marker to identify broodstocks of majalaya common
carp strain subsequently used as a candidate resistant to KHV infection. From a
total of 23 broodstock subjected to PCR analysis, two female and male fish,
both having (P) and no Cyca-DAB1*05 (N), were selected and then diallelly
mated. Disease resistance of progenies from 10 crosses was determined by a
survival analysis in pond rearing and a laboratory challenge-test using
cohabitation method. The results have revealed that the average survivals of
PxP progenies for pond rearing and KHV challenge test were 86% and 100% higher
(P < 0.05) respectively compared to that of NxN fish. Survival rate of
PxN/NxP progenies was significantly lower (P < 0.05) than that of PxP fish.
Furthermore, PCR analysis showed that almost 91% progenies of PxP crosses
seemed to have a KHV resistant gene marker. Thus, this study suggests that the
marker is associated with the KHV resistance in majalaya common carp strain,
and farming of PxP progenies can be useful to increase common carp production.
Keywords: Cyca-DAB1*05;
molecular marker; koi herpesvirus; Cyprinus carpio
Author: Alimuddin, Mubinun
Mubinun, Ayi Santika, Odang Carman, Irvan Faizal, Komar Sumantadinata
Journal Code: jpperikanangg110019
