IDENTIFICATION OF A LOCAL PROBIOTIC BACTERIUM USING 16S rRNA GENE SEQUENCE THAT WAS USED FOR FIELD TRIAL TO ENHANCED WHITELEG SHRIMP (Litopenaeus vannamei) SURVIVAL
Abstract: The use of local
probiotics in the culture of aquatic organisms is increasing with the demand
for more environmental-friendly aquaculture practices. The local bacterium
isolate considered as a probiotic was added into the water of whiteleg shrimp
(Litopenaeus vannamei) culture in a field trial. Four rectangular plastic ponds
(ca. 20 m x 30 m per pond) were used for 100 days experimentation for six
consecutive crops in two years experiment. Survival, harvest size, feed
conversion ratio (FCR) and Vibrio bacterial count was compared with those of
shrimp receiving and none of local isolate. Identification based on 16S rRNA
gene sequence shown those isolate was Bacillus pumilus strain DURCK14 with 99%
homology. Water shrimp pond added a local isolate had significantly higher
survival at about 10.0% to 11.7% than shrimp without added the isolate
(p<0.05), and better FCR, but no significant different in shrimp harvest
size. Vibrio bacterial was undetected by total plate count. Moreover, it shown
better projected yields on an annual basis (three crops per year).
Keywords: shrimp pond;
Litopenaeus vannamei; local probiotic; Bacillus pumilus; Vibrio; survival rate;
harvest size; FCR
Author: Tb. Haeru Rahayu,
Ketut Sugama
Journal Code: jpperikanangg150058
