GROWTH RESPONSE OF CLOWN LOACH (Chromobotia macracanthus Bleeker 1852) JUVENILES IMMERSED IN WATER CONTAINING RECOMBINANT GROWTH HORMONE
Abstract: The main problem in
the culture of clown loach (Chromobotia macracanthus) is the slow growth rate,
which takes about six months to reach its market size (two inches total body
length). Slow growth eventually cause a long production time and increase the
production costs. An alternative solution can be proposed in order to enhance
the growth is by using recombinant growth hormone. The aim of this study was to
determine the immersion dose of recombinant Epinephelus lanceolatus growth
hormone (rElGH) which can generate the highest growth in clown loach. Larvae at
seven day after hatching were hyperosmotic treated with NaCl 2.0% for one
minute, then immersed for one hour in water containing 0.3% NaCl, 0.01% bovine
serum albumin (BSA), and different doses of rElGH, namely: 0.12 (treatment A),
1.2 (B), 12 (C), and 120 mg/L (D). As control, fish were immersed in water
without rElGH and NaCl (control-1), water containing 0.3% NaCl and 0.01% BSA
(control-2), and 0.3% NaCl water (control-3). Each treatment was replicated
three times. The results showed that clown loach juveniles in treatment B, C,
and D had longer total body length (P<0.05) than control-1, while fish
treatment A was the same as controls. Survival and body weight were similar in
all treatments and controls (P>0.05). In addition, the percentage of large
size juveniles increased approximately 5% in treatment B, almost the same as in
the medium size, while the small size were decrease compared to the control-1.
Thus, the best immersion dose of rElGH was 1.2 mg/L water.
Keywords: growth hormone;
larvae; clown loach; immersion
Author: Asep Permana, Alimuddin,
Wartono Hadie, Agus Priyadi
Journal Code: jpperikanangg150056
