GENETIC VARIABILITY OF THREE POPULATIONS OF FLYING FISH, Hirundichthy oxycephalus FROM MAKASSAR STRAIT
Abstract: Flying fish,
Hirundichthy oxycephalus is one of economically important marine species to
Indonesia, particularly in Makassar Strait and Flores Sea. However, there is a
limited published data on genetic variation in molecular marker level of this
species. Random Amplified Polymorphic DNA (RAPD) was employed in this study to
determine the genetic variability of three populations of flying fish collected
from Takalar, Pare-Pare, and Majene in Makassar Strait. Genomic DNA was
isolated from preserved muscle tissue using phenol-chloroform technique. Two
selected arbitrary primers (CA-01 and P-40) were performed to generate RAPD
finger printing of flying fish populations. The two primers generated a total
of 81 fragments (loci) and 50 polymorphic fragments with size ranging from 125
to 1,250 bp. There were no significant differences in number of fragment and
number of polymorphic fragment among populations. The high polymorphism
(63.5±7.4%) was obtained from Takalar population followed by Pare-Pare
(58.3±19.6%) and Majene population (57.7±0.8%). Similarity index of individuals
was 0.60±0.17 for Takalar, 0.63±0.17 for Majene and 0.75±0.21 for Pare-Pare
population. Seven fragments were identified as species-specific markers of H.
oxycephalus. The UPGMA cluster analysis showed that the Takalar population was
genetically closer to Pare-Pare population (D= 0.0812) than to Majene
population (D= 0.1873).
Keywords: flying fish; genetic
variability; Makassar Strait; RAPD
Author: Andi Parenrengi, Andi
Tenriulo, Syamsul Alam Ali
Journal Code: jpperikanangg120023
