DISTRIBUTION AND EXPRESSION OF STRIPED CATFISH (Pangasionodon hypophtalmus) GROWTH HORMONE GENE (PhGH) IN THE ORGAN OF AFRICAN CATFISH (Clarias gariepinus) TRANSGENIC FOUNDER
Abstract: Faster growing
African catfish can be produced by transgenesis. This study was conducted to
investigate the distribution and expression of growth hormone gene (PhGH) in
various organs of the transgenic African catfish (Clarias gariepinus) founder
(F0). Transgene was detected using the PCR method in various organs, namely
pituitary, brain, liver, heart, spleen, kidney, intestine, stomach, muscle,
caudal fin, gill and eye. Transgene expression levels were analyzed using the
method of reverse transcriptase-polymerase chain reaction (RT-PCR), -actin gene used as internal controls. The results showed that the
PhGH was detected and expressed in all organs of the transgenic African catfish
founder. The high level of PhGH expression was found in the liver, pituitary,
intestine and brain; smaller amounts were detectable in muscle, spleen,
kidneys, heart, and stomach, caudal fin, gill and eyes, range from 0.02-0.75
PhGH/-actin mRNA. The expression levels of PhGH had
positive correlation with tissue and body size (P<0.05).
Keywords: gene expression;
transgenic; growth hormone; Clarias gariepinus; RT-PCR
Author: Huria Marnis, Raden
Roro Sri Pudji Sinarni Dewi, Imron, Bambang Iswanto
Journal Code: jpperikanangg120024
