FINE STRUCTURAL ANALYSIS OF UNPRODUCTIVE AND LOWPRODUCTIVE CELLS RESULTING ON CULTURED RSIV (IN VITRO)
Abstract: Red seabream
iridovirus (RSIV) has been known to induce enlarged cells and inclusion body
bearing cells (IBCs) allowing virus particles to propagate within viral
assembly site (VAS) in the cell cytoplasm. The aim of this study was to
evaluate fine structural analysis of unproductive and low-productive cells
resulting on RSIV-infected cultured grunt fin (GF) cells. GF cells were treated
with semi purified RSIV, and incubated for 6-14 days post cultured. The
cellular enlargement were harvested, processed, and analysis under electron
microscopy. Electron microscopy revealed four types of cells that were
productive enlarged cells and productive IBCs which were allowing propagation
of virus particles within its cytoplasm, and unproductive enlarged cells and
IBCs without virus particles. Most of them were unproductive enlarged cells
(80,71%-98,20%). Unproductive enlarged cell had a nucleus with enlarged
cytoplasm without production of VAS with virus propagation. While, unproductive
IBC had an inclusion body that was delimited from the host-cell cytoplasm by a
thin membranous boundary, and was developed without virus propagation. On the
other hand, lowproductive enlarged cells and IBCs contained a few number of
virus particles or tubule-like structures. Therefore, the number of
low-productive enlarged cells and IBCs were only a few (about 0%-14% from a
total of percent productive enlarged cells and IBCs), these cells were
classified into types of productive enlarged cells and IBCs. These results
sugested that the unproductive and low-productive enlarged cells and IBCs were
the results of RSIV-infected GF cells which failed to produce virus particles
due to incapacity of RSIV virus it self and or the ability of GF cells to
inhibit virus multiplication within VAS.
Keywords: RSIV; GF cells;
unproductive; low-productive; IBCs; enlarged cells
Author: Ketut Mahardika, Teruo
Miyazaki
Journal Code: jpperikanangg140052
