DIGESTIVE ENZYME ACTIVITY OF PROTEASE, α-AMYLASE, AND LIPASE OF RABBITFISH (Siganus guttatus) LARVAE
Abstract: One of the main
constraints in seed production of rabbitfish is the high mortality during
larval stage. Lack of suitable size of <150 mikron of live feed become the
main issue in hatchery because rabbitfish require very small feed, also
rabbitfish larvae have low capacity to digest the feed. The aim of this study
was to determine the activity of digestive enzyme including protease,
α-amylase, and lipase in rabbitfish larvae, as a basic for feed management.
Rabbitfish larvae were reared in two fiber glass tank filled with 700 L of
sewater (30-31 ppt salinity) and equipped with aeration. Test fish were newly
hatched rabbitfish larvae which were stocked at density of 50 fish/L. Live
feeds used were rotifers and Artemia nauplii. Measurement of the digestive
enzymes activity was conducted when the larvae age at 2, 5, 10, 15, 20, 25, 30,
and 35 days after heatching (DAH). The results showed that the highest enzyme
activity during endogenous phase was the protease of 0.0442 U/mL/minute and the
lowest was the α-amylase of 0.021 U/mL/minute. During exogenous phase, the peak
enzyme activity of protease occurred at 25 DAH, for the amylase occured in 15
DAH and for lipase 25 DAH. The enzyme activity was associated with age of the
rabbitfish larvae and related to the phase where larvae need to obtain life
feed.
Keywords: digestive enzyme;
rabbitfish larvae
Penulis: Kamaruddin, Usman,
Samuel Lante
Kode Jurnal: jpperikanandd150654
