ASSESSMENT OF GENETIC VARIATION OF PEARL OYSTER, Pinctada maxima, BASED ON THE ANALYSIS OF MITOCHONDRIAL CYTOCHROME OXIDASE SUBUNIT I GENE
Abstract: Pearl oyster,
Pinctada maxima is one of economical ly important species in aquaculture,
particularly in pearl industry. Information on genetic variation of pearl
oyster is required in order to be able to make a sound management of it’s
natural populations and to utilize it to improve the quality of pearl culture.
Five populations from different geographic locations of pearl oyster, Pinctada
maxima, (Sumbawa, Bali, Selat Sunda, Belitung, and South Sulawesi) were
analyzed for genetic variation within a 750-base pair region of the
Mitochondrial Cytochrome Oxidase subunit I (MtCOI) gene using Restriction
Fragment Length Polymorphism (RFLP) technique. The analysis of 25 pearl oyster
samples, their haplotype diversity ranged from 0.0970 to 0.1939 and the number
of haplotype in each population ranged from three to five haplotypes.
Clustering of populations based on Nei’s genetic distances and constructed
using unweighted pair-group method with Arithmetic mean (UPGMA) showed that the
populations were clustered into two groups: Belitung, Selat Sunda, Bali and
Sumbawa in one group, while South Sulawesi in the second group.
Keywords: genetic variation;
pearl oyster; Mitochondrial Cytochrome oxidase I (MtCO I)a
Author: Achmad Sudradjat, Rini
Susilowati, Imron
Journal Code: jpperikanangg090018
