AKTIVITAS ANTIOKSIDAN PADA FORMULA TABLET TERIPANG KELING (Holothuria atra)
ABSTRACT: This study about
activity of antioxidant aimed to determine the chemical characterization of
sandfish, sandfish meat and sandfish powder, determine the best formulation in
manufacturing Holothuria atra tablet based on DEPKES RI standard and to
determine the antioxidant activity and the active compounds that act as
antioxidants in the extract of Holothuria atra tablets. The method used in the
manufacture of tablets is the direct compression method using 6 formulations to
treat the kind of powder substance that is the ingredient of sandfish are
intact and substances from the meat of sandfish. Tablet chosen based on the
standards of the Ministry of Health that includes the physical characteristics
of the test tablet and shelf-life stability. Tablet rivet sandfishtested the
antioxidant activity by the method of 2,2-diphenyl-1-picrylhydrazyl (DPPH).
Tests to determine the active compounds that act as antioxidants with thin
layer chromatography (TLC) and bioautography antioxidants and also to determine
the functional groups using analysis of Fourier Transform Infrared (FTIR). The
yield of sandfish powder is considerably low. Yield of sandfish powder are
10.26% and sandfish meat powder are 9,82%. The protein content of sandfish
powder are 60,89% lower compared to sandfish meat powders of 63,57%. Formula
elected from the sandfish tablet test based on physical characteristics and
stability test of the shelf life of the tablet is the formula A2 with the
composition of the powder formulation Holothuria atra (25%), Ac-Di-Sol (2%),
L-HPC (2%), Aspartame (0.5%), menthol (0.2%), talk (1%), magnesium stearate
(1%) and lactose: mannitol (67.8%) and has antioxidant activity with IC50 value
of 97.22 ppm. TLC test and Bio-autography showed that the class of antioxidant
compounds are steroid, and suspected compounds at FTIR test, that act as
antioxidants compound has similarities to the sterol class.
Keywords: antioxidant,
Bioautografi, FTIR, Holothuria atra, sandfish, stability, tablets, TLC
Penulis: Ahmad Fauzan Lubis,
Sri Purwaningsih, Kustiariyah Tarman
Kode Jurnal: jpperikanandd160493
