VARIASI SEKUENS DNA YANG DIAMPLIFIKASI MENGGUNAKAN PRIMER atpB-rbcL PADA BEBERAPA KULTIVAR KACANG TANAH
Abstrak: Peanut is one of food
crops commonly consumed in Indonesia. This species comprises several cultivars
such as Kancil, Bison, Jerapah, Talam, and Tuban, each of which has its
individual advantages and disadvantages. The vast variation among peanut
cultivars leads to the need of study on genetic diversity and relationship
among them using particular molecular marker. This study aims to see whether
variation on DNA sequences among some peanut cultivars amplified with atpB-rbcL
primers exists or not and to know the relationship among the cultivars based on
the amplicon sequences. The method involves some sequential steps, i.e. genomic
DNA isolation using CTAB protocol, amplification of DNA sequence using
atpB-rbcL primers and sequencing of the amplification products. Data on
sequences were edited manually using Bioedit version 7.0.4.1. Sequence
alignment was performed using ClustalW, which is also implemented in Bioedit
version 7.0.4.1. Arlequin 2.0 was used to calculate nucleotide diversity p.
Phylogenetic analysis was performed using Maximum Parsimony in MEGA 5.0. The
results showed that considerably high variation in DNA sequences of some peanut
cultivars amplified with atpB-rbcL primers are observed. On the other hands,
very close genetic relationship among cultivars is found.
Penulis: Yani Yuliani, Alice
Yuniaty, Agus Hery Susanto
Kode Jurnal: jpbiologidd170018
