KERAGAMAN GENETIK 24 VARIETAS PADI SAWAH DAN PADI GOGO (Oryza sativa L.) INDONESIA BERDASARKAN MARKA SSR
Abstrak: The use of molecular
marker is an efficient approach to analyze the genetic diversity and it can be
used widely in biological studies. The characterization of rice germplasms by
using molecular markers technique is more precise because it is not influenced
by environmental factors. The purpose of this study was to analyze the genetic
diversity of 24 varieties of lowland and upland rice by using 15 SSR markers.
The results showed as many as 86 alleles were detected in 24 rice varieties,
with the average number of alleles per marker was 5.73 and the range of alleles
per locus was 2-10. The average of major allele frequency was 43% with the
lowest score was 26% on RM6997 and RM536 markers and the highest score was 65%
on RM60 marker. A total of 14 SSR markers were able to discriminate
heterozygous alleles within a range between 0.17 (RM105) to 1.00 (RM201, RM263,
RM416, RM518 and RM223). The value of gene diversity ranged from 0.48 (RM60) to
0.81 (RM536) with an average of 0.70. The value of PIC (Polymorphic Information
Content) ranged from 0.38 (RM105) to 0.78 (RM536) with an average of 0.65. The
phylogenetic analysis showed that 24 rice varieties separate into two main clusters
in the coefficient of 0.63. The first cluster consists of 12 lowland varieties
and the second cluster consists of 12 upland varieties. The genetic diversity
data in this study were expected could be a valuable information in the rice
plant breeding activities in the future.
Penulis: Kristianto Nugroho,
Slamet, Puji Lestari
Kode Jurnal: jpbiologidd170017
