TOTAL FENOL DAN AKTIVITAS ANTIOKSIDAN DARI SONNERATIA ALBA BARK
ABSTRACT: The aim of this
research was to examine the total phenolic content and radical scavenging
capacity related to antioxidant potential in different fractions of methanolic
extract of mangroves trees, Sonneratia alba bark. Total phenolic content was
determined by the Folin-Ciocalteau
method. Antioxidant potential
has been determined as
the free radical scavenging
ability using a stable radical, diphenyl-picryl-hydrazyl (DPPH). Three
fractions of methanol extracts of S.
alba were subjected to free radical scavenging activity in order to evaluate
their antioxidant activity. The free radical scavenging activity of different
fractions obtained from successive fractionation of methanol extracts with
organic solvents of different polarities; hexane, chloroform, and ethyl
acetate. Total phenolics content of three fraction and crude extract are 17.84
±0.974, 156.310 ±0.703, 226.89 ±0.605, 249.56
±0942 (GAE μg/g) respectively for hexane, chloroform, ethyl acetate, and
crude extracts (methanol extract). Antioxidant assay showed that the ethyl
acetate and chloroform fractions have the high activity with IC50 10.27 μg/ml
and 27.34 μg/ml, respectively, while a
weak activity was showed by hexane fraction with 147 μg/ml. Linear correlation
between the total antioxidant capacity of the fractions and the total phenolic
contents was observed (r2=0.918). This data indicated that the antioxidant
activity mainly due to phenolic compound
contain in those fraction. The results indicate promising S.alba or the
utilization as significant source of natural antioxidant.
Penulis: Netti Herawati
Kode Jurnal: jpkimiadd120417
