Klon-klon Kentang Transgenik Hasil Persilangan Terseleksi Tahan terhadap Penyakit Hawar Daun Phytophthora infestans Tanpa Penyemprotan Fungisida di Empat Lapangan Uji Terbatas
ABSTRACT: The use of resistant
varieties is an appropriate alternative in controlling the late blight, a major
diseases on potato, caused by the fungus Phytophthora infestans. The
development of late blight resistant potato was done through hybridization
between non-transgenic Atlantic or Granola with RB transgenic Katahdin SP904
and SP951. The hybrid clones which have been positively contained the RB gene
were evaluated for the resistance to P. infestans in four Confined Field Trials
(CFTs) i.e. Pasir Sarongge (2008), Lembang (2009-2010), Pangalengan (2010-2011)
and Banjarnegara (2011-2012). There are twelve selected hybrid clones which
were resistant to P. infestans both in each location of CFT or in four
locations were obtained. These clones consist of five clones from crosses of
Atlantic and trangenic Katahdin SP951 (B35, B169, B163, B11, B162) and seven
clones from crosses of Granola and transgenic Katahdin SP951 (D76, D12, D25,
D48, D38, D37, D15). The selected hybrid clones showed resistance to P.
infestans until 14 to 18 days after infection or about 40 to 45 days after
planting, in the absence of fungicide spraying. The hybrid clones had a resistance
score varied from 7,65 to 8,23 and were significantly different from the
parents Atlantic and Granola, with a resistance score of 3,6 and 3,45,
respectively. This was also supported by AUDPC values, which showed that AUDPC
of the hybrid clones were in the range between Atlantic or Granola and
transgewnic Katahdin SP951. This indicate that the resistance level of the
hybrid clones is in the range between susceptible and resistant check. The
resistant hybrid clones are valuable genetic resources for late blight
resistance breeding programs, particularly in reducing the frequency of fungicide applications.
Penulis: Alberta Dinar
Ambarwati, Kusmana, Edy Listanto
Kode Jurnal: jpbiologidd150833
