KARAKTERISASI KULTUR MAKROFAG HASIL ISOLASI Mouse Peritoneum Makrofag (MPM)
Abstract: The aims of this
study is to determine the MPM isolation techniques, results of the isolation
and identification, so it can be used as a reference for the studies using
macrophage cell cultures. The most appropriate source for culturing macrophages
are the primary culture of MPM. Resident peritoneal macrophages (RPM) is a
free-living phagocytic cells in the peritoneal cavity. Millions of resident
macrophages can be taken from the mice. The peritoneal cavity is an easy place
to harvest the resident macrophages. Identification of macrophages was
conducted using flowcytometry with specific marker CD 11B. While the morphology
of macrophages in culture was observed microscopically The results of the study
showed the average number of macrophages 1.03 x 106 cells / ml were isolated
from peritoneal cavity of mice. The result of identification with flowcytometry
with marker CD 11 B shows positive results macrophages. While the observation
of morphological characteristics of macrophages cultures show the same characteristics
as the result of research conducted by Kim in 1997 and Wang et al, 2010 so that
it can be concluded that macrophages can be isolated from the peritoneal cavity
and can be used as a source of primary cultures of macrophages with good
results and can be applied to studies related to the function and activation of
macrophages in vitro.
Penulis: Erna Susanti
Kode Jurnal: jpbiologidd150966
