Induksi Tunas in vitro Jeruk Siam (Citrus nobilis Lour.) Asal Kampar pada Berbagai Konsentrasi Sukrosa
Abstract: This study aimed to
determine the most effective concentration of sucrose for a shoot induction
fromcotyledon explants of Citrus nobilis from Kampar. Cotyledon explants were
cultured on Murashige andSkoog (MS) medium containing sucrose at various concentrations
without supplemented with growthregulators. The treatments consisted of five
concentrations of sucrose, i.e. 0, 20, 30, 40, 50, 60 g /l. Theresult showed
that all explants on different concentrations of sucrose treatment had the
percentage ofliving and the percentage of shoot induction reached 100%. The
best response for shoot induction aftereight weeks of culture was observed on
MS medium supplemented with 50 g/l sucrose with the number ofshoot was 2, shoot
length was 4,125 cm, and leaf number was 5,74 strands.
Penulis: SITI FATONAH, MAYTA
NOVALIZA ISDA, WAHYU LESTARI
Kode Jurnal: jpbiologidd160579
