Induksi PLB Anggrek Vanda sumatrana Schltr. Liar Pada Media MS dengan Penambahan BAP dan NAA serta Ploidisasi dengan Kolkisin
Abstract: The study about PLB
induction of wild Vanda sumatrana Schltr. on MS media suplement with BAP and
NAA and ploidisation by colchicine treatment was conducted from December 2014
until November 2015 at the Laboratory of Genetics and Cell Biology and
Laboratory of Plant Physiology and Tissue Culture, Biology department, Faculty
of Mathematic and Natural Science, Andalas University, Padang. The study aimed
to 1) knowing the best concentration of 6-Benzyl amino purin (BAP) and
α-Naphtalene acetic acid (NAA) for Protocorm Like Bodies (PLB) induction from shoot tip of V. sumatrana, 2)
knowing the PLB response of V. sumatrana to concentrations and soak period of
colchicine and 3) find the effective concentrations and soak period of
colchicine to induce tetraploid on PLB of V. sumatrana. Shoot tips from
in-vitro cultured of V. sumatrana were
subcultured on Murashinge and Skoog (MS) medium supplement with 3 mg/l BAP +
0,5 mg/l NAA, 3 mg/l BAP and 1,5 mg/l BAP. PLB of diploid V. sumatrana from the
best treatment were soaked in 0.05% and 0.1% colchicine for 24 and 48 hours
respectively in MS liquid medium, as control were set PLB without colchicine
treatment. The results showed that MS medium supplemented with 1.5 mg/l BAP was
the best formula to induce PLB. The highest percentage of survival rate of PLB
and percentage of survived PLB regenerated shoot was obtained from 0.05%
colchicine with 24 hours soak period treatment. The effective treatment to induce
tetraploid on PLB of V. sumatrana Schltr. was obtained from 0.05% colchicine
solution for 24 hours soak period.
Penulis: Hanifah Aini,
Mansyurdin, Suwirmen
Kode Jurnal: jpbiologidd150849
