Induksi kalus Artemisia vulgaris L. dengan Pemberian Beberapa Konsentrasi 2,4-Dichlorophenoxyacetic Acid(2,4-D)
Abstract: The research about
callus induction Artemisia vulgaris L. by giving several concentration
2,4-Dichlorophenoxyacetic acid (2,4-D), has been done from May to August 2015
in Plant Physiology Laboratory and Tissue Culture, Department of Biology,
Faculty of Mathematics and Natural Science, University of Andalas. The aim of
this studywas found the effective concentration of 2,4-D to induce callus of A.
vulgaris. The research used Completely Randomized Design (CRD) with 7
treatments and 4 replications. The treatments were : without 2,4-D (control);
0.25 mg/L 2,4-D; 0.50 mg/L 2,4-D; 0.75 mg/L 2,4-D; 1.00 mg/L 2,4-D; 1.25 mg/L
2,4-D; 1.5 mg/L 2,4-D. The result showed that 0.25-1,5 mg/L 2,4-D were able
induction callus of A. vulgaris, withcompactuntilthefriabletexture, color ofthe
resultingcallusisyellowish green, brownish-green, yellow-brown,
whiteyellowishandgreenishwhite. 2,4-D 1.5mg/Lwas the best concentrationto
increase fresh weight of callus.
Penulis: Nazhira Fadhilah,
Zozy Aneloi Noli, Suwirmen
Kode Jurnal: jpbiologidd150849
