DESAIN PRIMER PCR SECARA IN SILICO UNTUK AMPLIFIKASI GEN COI PADA KUPU-KUPU Papilio ulysses Linnaeus DARI PULAU BACAN
Abstract: The insillico
research was conducted to produce primer sequence of COI gene in Butterfly
species Papilio ulyses from Bacan Island that will be used in gene isolation.
The COI sub-unit I is a barcode gene that generally have special purposes in
animal identification. It is also available in phyilogenetic analysis. The
first step of method is downloading the COI DNA sequence of Papilio ulysses
from GeneBank (NCBI). Then the conserve region of COI sequence is identified to
determine primer. Primer is designed with primer designer software (Genamics
Expression) in two direction of DNA reading frame that are : forward primer
(COI Pu-F) and reverse primer (COI Pu-R). The last step is primer confirmation
with primer blast tool in NCBI. Primers of COI gene that are produced consist
of two, that are CGAAAATGACTTTATTCAACA for forward primer (COI Pu-R) and
AGCAGTAATTCCAACAGCTC for reverse primer (COI Pu-R). The optimal temperature of
annealing is from 50,740 to 55,740 Celsius with PCR product around 567 base
pair long.
Penulis: Suparman
Kode Jurnal: jpmatematikadd160270
