Characteristics of Pigeon Pea (Cajanus Cajan) Isoflavones Daidzein in Blood on Ovarian And Mammary Tissue Structure Rat Female
Abstract: The use of synthetic
hormone preparations, one of the hormones estrogen widely used by people in the
care and treatment of diseases as
hormone replacement therapy. Pigeon pea (Cajanus cajan) as local Leguminoceae
plant has not been used optimally as estrogenic plants . The purpose of this
study was to identify preclinical to analyze the Cajanus cajan in the blood
profile and potential of the reproductive organs as phytoestrogens. The
research method using 24 Sprague -Dawley female rats 6-7 months , grouped 3
treatment. The control group , the treatment group pigeon pea solution of 1 : 1
and group treatment solution pigeon pea 1 : 3. Blood sampling on the 8th, 16th
hour and the 24th hour (fractions I, fractions II and fractions III ) for the
analysis of isoflavones daidzein HPLC method . Treatment pigeon pea solution
made up to 36 days. Surgery and organ harvesting ovarian and mammary continued
hematoxylin eosin staining . The results showed levels of isoflavones daidzein
maximum in treatment 1 : 1, fractions I, II , and III respectively 92773.474 pg
/ l ; 96766.278 pg / l ; 72033.062 pg / l. Tissue structure experiencing
development of ovarian follicles and
germinal proliferation, proliferation of mammary tissue experiencing the
lobules and ducts interlobularis development. Conclusion of the study is the
pigeon pea daidzein levels in the blood increased in the 24th hour and
potential as a natural estrogen.
Penulis: Cicilia Novi
Primiani, Pujiati
Kode Jurnal: jpbiologidd160250
