UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOLIK DAGING BUAH BUNI (Antidesma bunius (L.) Spreng) TERHADAP Staphylococcus aureus ATCC 25922 dan Escherichia coli ATCC 25923
Abstract: The aim of the study
was to determine the antibacterial activity of buni skin-pulp ethanolic extract
against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922.
Profile of antibiotic resistance which is growing among Staphylococcus aureus
and Escherichia coli need some exploration of antibacterial activity buni
skin-pulp because the contain of anthocyanin which has antibacterial activity.
The research was purely experimental research with randomized complete direct
sampling design. The extraction method was done by macerated in ethanol
solvent. Tube tests and Thin Layer Chromatography (TLC) were used to determine
the content of the secondary metabolites substance in buni skin-pulp ethanolic
extract. Antibacterial activity test was done by diffusion method, then
followed with liquid dilution method to determine the Minimum Inhibitory
Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The
antibacterial activity was evaluated based on the result of inhibition zone
diameter then analyzed with Kruskal-Wallis test followed with Mann-Whitney
test. The results showed that the chemical substances of the buni skin-pulp
ethanolic extract predicted with TLC assay were phenolic, flavonoids, and
anthocyanin compounds. The antibacterial activity showed that the ethanolic
extract only had antibacterial activity against Staphylococcus aureus ATCC
25923 with MIC and MBC values 30% and 33%, respectively.
Penulis: Brigitta Lynda
Rakasiwi, C.J. Soegihardjo
Kode Jurnal: jpfarmasidd140399
