IMMUNOSUPRESSANT EFFECT OF THE EXTRACT OF Tridax procumbens ON LYMPHOCYTE CELLS LINE FROM PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOUS: IMMUNOGLOBULIN GBIOMARKER
ABSTRACT: Systemic Lupus
Erythematous (SLE) is one of the major autoimmune diseases that can cause severe
damage to the tissues and organs in the body and lead to high mortality rates.
The clinical manifestations are characterized by the production of autoantibody
immunoglobulin G (Ig G) that fights component of the nucleus. The present
scientific information issues about peculiar property of Tridax procumbens as
immunosuppressant by inhibiting enzyme of cyclooxygenase-2 (COX-2) on surface
of B cells. This study approved effect of the extract of Tridax procumbens on
human lymphocytes cells line with SLE disease. The aim of this study was to
approve effect of extract of Tridax procumbens in suppressing Ig G
concentration from patient with stable SLE and patient with active SLE in
vitro. This study was an in vitro experimental design on lymphocytes cell line
that was isolated from whole blood patient withstable SLE and active SLE.
Lymphocyte cells from healthy volunteer were also collected as control.
American Rheumatism Association Diagnostic was used for inclusion
criteria.Tridax procumbens was extracted with maceration method in solution of
ethyl acetate. The extract was divided in several doses as immunosuppressant
i.e.1000; 250; 62.5; 31.25; 15.13; and 3.91 µg/mL. Supernatant of the cultured
cells was collected and examined for measuring Ig G concentration as secretion
from activated B cells by ELISA. Ethical clearance had been completed before
this study started. The extract of Tridax procumbens significantly suppressed
Ig G secretion in patient with stable SLE at dose over 62.5 µg/mL, but Ig G concentration
in patient with active SLE was significantly increased when compared with control.
Furthermore from the analysis, effect of the Tridax procumbens extract on Ig G concentration
followed dose dependent response. In in-vitro study, the extract of Tridax procumbens
could significantly suppress Ig G secretion in healthy volunteer and patient
with stable SLE. Otherwise, the extract does not suggest for patient with
active SLE and it needs caution in use and investigation in-depth.
Penulis: Abdul Khairul Rizki
Purba, Endang Mahati, Yolanda Dyah Kartika, Indwiani Astuti, Nyoman Kertia
Kode Jurnal: jpfarmasidd130260
