ANALISIS KUANTITATIF ISOFLAVON TEMPE SECARA CEPAT DAN SEDERHANA MENGGUNAKAN METODE KROMATOGRAFI LAPIS TIPIS- DENSITOMETRI
Abstract: Rapid and simple
quantitative analysis isoflavones tempe using densitometric TLC has been done.
The mobile phase of the system was chloroform: methanol: ethylacetate (45: 5:
0.75). Thin layer chromatography was performed on aluminium TLC
plates.Ascending distance of 1 μL sample was performanced 10 cm. Then the plate
was scanned at 261 nm. A linear relationship obtained at 0.08 - 2 μg/spot with
r= 0.9986. The LOD and LOQ of isoflavone were 0.014 μg/spot and 0.048 μg/spot.
Genistein contained in tempe was 0.151± 0.005 % b/b.
Penulis: Agustina Setiawati,
Sri Hartati Yuliani, Enade Perdana Istyastono, Michael Rahardja Gani, Evy Fenny
Veronica, Dina Christin Ayuning Putri, Reza Eka Putra, David Chandra Putra,
Agnes Mutiara Kurniawan
Kode Jurnal: jpfarmasidd140410
