POTENSI Bacillus licheniformis DAN Streptomyces olivaceoviridis SEBAGAI PENGHAMBAT PERTUMBUHAN JAMUR Saprolegnia sp, PENYEBAB SAPROLEGNIASIS PADA IKAN SECARA IN VITRO
Abstract: Saprolegniasis is a
fungal disease on fish caused by Saprolegnia sp, which is saprophyte, damaging
healthy tissue and makes the immune system in fish deacreased. The uniquennes
of Saprolegnia sp has the main components of the cell wall in the form of
chitin that was instrumental in shaping the structure of the tip growth of
fungal hyphae. Control of fungal pathogenic Saprolegnia sp can use chitinolytic
microorganisms based on ability to produce chitinase for example using
bacteria. This study aims to potential of Bacillus licheniformis and
Streptomyces olivaceoviridis as inhibiting the growth of fungus Saprolegnia sp,
cause saprolegniasis on fish by using in vitro. The research method is
experimental with completely randomized design (CRD), which consists of four treatments
with five replications. The experimental used is A (Saprolegnia sp and Bacillus
licheniformis), B (Saprolegnia sp and Streptomyces olivaceoviridis), C negative
control (Saprolegnia sp) and D positive control (Saprolegnia sp and Ketokonazol
2 %). The main parameters measured were observed inhibition zone on each
treatment. Supporting parameters were observed is an observation of abnormal
hpyphae structure after being induced by bacteria Bacillus licheniformis dan
Streptomyces olivaceoviridis. Data were analyzed using analysis of variants
(ANOVA) and to know the difference between treatments were determined by Tukey
honestly significant difference (Tukey HSD) Test. The results showed that the
potential of chitinolytic Bacillus licheniformis can provide a good barrier of
4,62 cm by 5,48 cm compared Streptomyces olivaceoviridis in inhibiting the
growth Saprolegnia sp. Suggestions in this research is the need to further
research on the value of chitinase Bacillus licheniformis and Streptomyces
olivaceoviridis. Further research by using in vivo.
Penulis: Oktantia Frenny
Anggani, Rahayu Kusdarwati dan Hari Suprapto
Kode Jurnal: jpperikanandd150172
