The effect of Different Media Content on Protease Activity Bacillus subtilis
ABSTRACT: Enzymes were protein
molecules that synthesized cells to accelerate biochemical reactions. One of
the microorganisms that produce a protease was Bacillus subtilis. B. subtilis
used in this research consisted of isolate 1 and isolate 2. Methods used
include thequalitative assay of protease activity through theclear zone on skim
milk agar and calculation of protease activity on production media such as NB
(Nutrient Broth) and TSB (Tryptic Soy Broth). Based on the results revealed
that the clear zonediameter isolate 2 better than the isolate 1 after 24hours
and 48 hours incubation periods. It showed both isolates having the possibility
in different strains but within a species. The highest protease activity
calculation obtained from TSB production media respectively 0,14 Unit /ml unit
and 0.12 Unit/ml.
Author: Siti Nurkasanah,
Widodo
Journal Code: jpbiologigg150009
