Effectivity Combination of Bitter Melon (Momordica charantia) and Bitter (Andrographis paniculata) Extract to Suppress Proinflammatory Cytokines in Diabetic Mouse Models
ABSTRACT: Insulin Resistance
(IR) is main characteristic of Type 2 Diabetes Mellitus. IL-6 and IFNγ play
important role in the deterioration of this disease. The aim of this experiment
was to know effectivity combination of Bitter Melon and Bitter to decrease the expression
of IL-6 and IFNγ in Type 2 Diabetes Mellitus. Type 2 Diabetes Mellitus were
induced by injecting streptozotocin to neonate BALB/c mice in the age of five
days (100 mg/kg BW). This experiment applied five groups which divided into
normal group, T2D (Type 2 Diabetes Mellitus model group), T2D-D1 (Bitter Melon
dose 5,6 mg/kg BW and Bitter 20 g/kg BW), T2D-D2 (Bitter Melon doses 56 mg/kg
BW and Bitter doses 200 mg/kg BW) and T2D-D3 (Bitter Melon doses 5600 mg/kg BW
and Bitter doses 20000 mg/kg BW). Relative number of proinflammatory cytokines
were analyzed using One-Way ANOVA with SPSS 16.0 for Windows. The results
showed that the treatment with Bitter melon (Momordica charantia) and bitter
(Andrographis paniculata) gave different effect compared to T2D groups.
Medicinal herbs groups supressed proinflamatory cytokines such as IL-6 and
IFNγ. So that relative number of IL-6 and IFNγ in treated group is lower than
Type 2 Diabetes Mellitus (T2D) mice model.
Author: Firda Agustin and
Muhaimin Rifa’i
Journal Code: jpbiologigg150008
