Respon Eksplan Nodus dan Daun Tanaman Binahong (Anredera cordifolia L.) pada Media MS dengan Variasi Konsentrasi BAP
Abstract: The purpose of this
study was to determine the response of nodal explants and leaves of plants
binahong on MS medium with the addition of 2,4-D with BAP variation. Research
was designed using two factors: factor 1: types of explants (E1 = node, E2 =
leaf), factor 2: the concentration of
plant growth regulator BAP (B0 = 2,4-D 2 mg / L (without BAP); B1 = 2, 4-D 2 mg
/ L and BAP 1 mg / L; B2 = 2,4-D 2 mg / L and BAP 2 mg / L). Explants nodes and
leaves of plants Binahong sterilized using Bayclin 45 "sterile distilled
water and then rinsed 3 times
further incubated in
a culture room.
Parameters include callus
formation time (Days
After Planting / HST), size, and color callus. Additional parameters:
the presence or absence of roots and shoots. Observations made during the 35
days and the result that the treatment of E1 (explants nodes) more quickly induced
to form callus and callus size larger than E2 (leaf explants). B0 treatment
induced to form roots (E1 and E2), while B1 forming buds at E1. The conclusion
is the response of nodal explants Binahong better for the formation of callus
on MS medium with the addition of 2 ppm 2,4-D and variations in the
concentration of BAP (0, 1, 2 ppm) compared explants of leaves and the most
excellent in the treatment E1B1 (explants nodes on MS + 2 ppm 2,4-D and 1 ppm
BAP).
Penulis: Triastuti Rahayu,
Ucik Mardini
Kode Jurnal: jpbiologidd150515
