PRODUKSI BIOETANOL DARI SAMPAH DEDAUNAN SEKITAR KAMPUS UNAND DENGAN METODE SSF (SIMULTANEOUS SACHARIFICATION FERMENTATION)
ABSTRACT: In this research,
cellulose from waste leaves are conversed into ethanol by SSF technology using
cellulase from Trichoderma viride strain T1 sk. In saccharification process,
cellulase breaks cellulase polymer into glucose. Simultaneously, the formed
glucose is conversed into ethanol by invertase produced by Saccharomyces
cerevisiaewhich is grew on YPD medium. Waste leaves are pretreatmented using
basic solutions : basic NaOH 1 %, NH 4 OH 8 %, NaOH 1 % + NH 4 OH 4 % and NaOH
1 % + NH 4 OH 8 % with ratio of solid mather (sample) : liquid (basic solution)
1:10 (w/v) with volume of basic solution 100 mL. Immersion time is variated for
24, 48 and 72 hours on 50 0 C. The result of research shows that the use of
NaOH 1% + NH 4 OH 4% gives the highest glucose concentration 933,75 µg/mL with
immersion time for 72 hours on 50 0 C. After being pretreatmented, sample of
0,4 g waste leaves produce the highest glucose concentration. Measurement by
GC/MS shows ethanol concentration 62,41% on fermentation time 96 hours with
volume of ethanol 2,45 mL for 0,4 g sample.
Penulis: Marniati Salim, Elida
Mardiah,Yollanda Atmelwidia
Kode Jurnal: jpkimiadd140696
