PENGGUNAAN SUMBER NITROGEN TERHADAP KANDUNGAN PROTEIN DAN ASAM AMINO PADA MIKROALAGA Spirulina platensis
Abstract: Through an
experiment process, the
usage of nitrogen
source to protein
and amino acid content
in microalgae Spirulina platensis has been found. This research aimed to
analyze protein and amino acid content in Spirulina platensis cutured in two
mediums; medium with NaNO3 as nitrogen
source and urea
as nitrogen source,
and to determine
the best nitrogen
source in Spirulina platensis
growth. Protein extraction
performed in alkaline
extraction method for 5 hours and
followed by precipitation
in pH 3.
Amino acid analysis
was performed with Aminoacid Analyzer.
Protein content determination
was carried out
using Bradford method that
shows the highest
protein content in
urea biomass culture
is 768.17 ppm.
Sodium nitrate biomass cuture
shows the lower
protein content, that
is 146.4 ppm.
The growth of
Spirulina platensis was done with spectrophotometer at 560 nm. Amino acid analysis shows that NaNO3 biomass
culture yield the higher amino acid content than urea, but urea biomass culture
shows the best growth with the higher absorbant than NaNO3 biomass culture.
Penulis: Magistrina Prima
Putri, Sumaryati Syukur, dan Zulkarnain Chaidir
Kode Jurnal: jpkimiadd150280
