AMPLIFIKASI GEN 16S-rRNA BAKTERI TERMOFILIK DARI SUMBER AIR PANAS, GUNUNG PANCAR BOGOR
ABSTRACT: Exploration of
thermophilic bacteria that produce thermostable enzyme is most useful in application
for enzyme base industrial. The aim of of this research is to isolate and
amplificate the 16S-rRNA gene from thermophilic bacteria isolate at hotspring,
Mount of Pancar, Bogor. The research steps consist of bacteria isolation,
chromosomal DNA extraction, and amplification of 16S-rRNA gene. The water
sample as source for bacteria was collected from four cauldrons. Temperature
and pH for each cauldron are red cauldron 75-80°C, pH 7; black cauldron 55°C,
pH 7; white cauldron 57°C, pH 7; and saline cauldron 25°C, pH 6, respectively.
The bacteria were cultivated at Luria Bertani (LB) and Thermus media. The
chromosomal DNA have been extracted. Gene amplification of 16 S-rRNA have been
carried out by using universal primer (Bac F1 and Uni B1). The size of amplicon
is ± 1.5kb.
Penulis: Suryani, Laksmi
Ambarsari, Efi Sanfitri Harahap
Kode Jurnal: jpkimiadd090198
