Peluang Gen rbcL sebagai DNA Barcode Berbasis DNA Kloroplas untuk Mengungkap Keanekaragaman Genetik Padi Beras Hitam (Oryza sativa L.) Lokal Indonesia
Abstract: Black rice (Oryza sativa L.) is one of germ
plasm where there are many found in Indonesia. The popularity of black rice
growing in line with public awareness for consuming a functional food. But the
scientific study of black rice germ plasm in Indonesia still classified as low,
especially the scientific study of the molecular genetik diversity. Information of molecular
genetik diversity adequately on black
rice germ plasm is one of the
cornerstone for the
development of action
strategies for conservation
the varieties of
black rice in Indonesia in a sustainable way. The scientific study which is expected to be implemented in the
form of research on black rice molecular
genetik diversity is an approach
of DNA barcode. Most of the genes that utilized as
DNA barcode on
plants is on
chloroplasts DNA (cpDNA).
cpDNA having the
benefit characteristics for
genetik diversity study compared
with nuclei DNA (nDNA ) and mitochondria mtDNA. Characteristic of these is (1)
the cpDNA genome have a small size and stable structure, (2) More conservative with
lower average nucleotide substitution and (3) no recombination and uniparental
heritable. This article discussed a potential DNA sequences of nucleotides from
chloroplast DNA to give information on
the genetik diversity of black rice, namely rbcL gene. Until now, NCBI database
collected 143 partial sequence of rbcL gene. The number of partial sequence
that has been collected is using as comparation facilitate to investigation the genetik
diversity of Indonesia black rice. In the future, DNA barcode os
expected to be implemented in the research.
Penulis: Abdul Basith
Kode Jurnal: jpbiologidd150318
