Kerusakan Sel Hepar Tikus Putih Jantan (Rattus norvegicus) yang di Induksi Karbon Tetraklorida (CCl4) setelah Diberi Ekstrak Etanol Bawang Dayak (Eleutherine palmifolia Merr.)
Abstract: The purpose of this study is to determine the
effect of giving Bawang Dayak extract as
a hepatoprotector to CCl4 induced mice. The research design used in
this study was experimental research design by using 25 white
male mice aged 2-3 months as samples. The ethanol extract of Bawang Dayak was
given orally and induce by using intraperitoreal injection, so the treatment was divided into
5 groups; group 1) negative control (without treatment), 2) positive control
which was induced CCl4 at a dose 1.3 ml/kg BW ip, 3) given Bawang Dayak extract
at a dose 90 mg/kg BW po + CCl4 induced at a dose 1.3 ml/kg BW ip, 4) given
Bawang Dayak extract at a dose 100 mg/kg BW po + CCl4 induced at a dose 1.3
ml/kg BW ip, 5) given Bawang Dayak extract at a dose 110 mg/kg BW po + CCl4
induced at a dose 1.3 ml/kg BW ip. The treatment was done for 53 days. On the
54th -57th day, the mice were injected CCl4 intra
peritoneal. On the 58th day, the mice were dissected and taken the
hepar organ. After that, making the hepar preparat with safranin staining was
done. The observation was done by looking at the damaged hepar cells which were
classified into piknotik, main karioreksi and kariolisis from 3 fields of view.
The data was analyzed by Anova One Way. The result of the study showed the
average score of hepar damage of group 1=48, group 2=107.4, group 3=89.2, group
4=61.8, and group 5=45. The analysis showed that giving Bawang Dayak extract at
a dose 110 mg/kg BW could improve hepatoprotector CCl4 induced mice.
Penulis: Aulia Risqi Rohmatin,
Eko Susetyarini, Samsun Hadi
Kode Jurnal: jpbiologidd150317
