Konstruksi Mutan Pseudomonas sp. untuk Meningkatkan Produksi Indole Acetic Acid (IAA) melalui Mutagenesis dengan Transposon
Abstrak: Pseudomonas sp. is
one of bacterial groups having ability to promote plant growth and health. Of a
hundred successfully isolated Pseudomonas sp. from soybean rhyzosphere, 98 were
found to produce indole acetic acid (IAA) ranging from 0.33 to 16.02 ppm. These
isolates are bacilli, motile, Gram negative, and showing positive oxidase
assay. One of them, i.e. Pseudomonas sp. CRB17, can promote plant growth by
means of significant stimulation of primary root length and lateral root
number. This isolate was then subject to mutagenesis using transposon
Mini-Tn5Km1 to increase IAA production. Mutagenesis was done by conjugation
between E coli S17-1 (l pir) carrying transposon mini-Tn5Km1 (donor) and
Pseudomonas sp. (recipient), resulting in conjugation frequency of approximately
3.1 x 10-5 cell per recipient. The resulted CRB17 mutants were then tested for
their ability to produce IAA, one of which showed an increment of IAA
production up to 77.5%, while some others showed no significant change or even
had a reduction to 55.3%. Sequence
analyisis of 16S rRNA gene of Pseudomonas sp. CRB17 indicated that it has a
high homology with that of Pseudomonas plecoglossicida (identical value of
99%). The results recommends that mutagenesis using transposon can be applied
to increase IAA production, especially in Pseudomonas sp. CRB17.
Penulis: Aris Tri Wahyudi,
Mutiha Panjaitan, Nisa Rachmania
Kode Jurnal: jpbiologidd090075
