Identifikasi dan Karakterisasi Isolat 23 Ducc Serta Kemampuannya dalam Memproduksi Enzim Inulinase
Abstrak: Inulinase has a
capacity to convert inulin into fructose hence it could be used in high
fructose syrup production. Inulinase is not readily available in Indonesian
market. This enzyme could be obtained from thermotolerant yeast found in Dahlia
variabilis. Therefore this study was conducted to identify inulinolytic yeast
in D. variabilis. The results showed
that an inulinolytic yeast isolate 23 DUCC from D. variabilis tubers was
found. The inulinolitic yeast produces inulinase (E.C.3.2.1.7). Based on the
identification and determination, the isolate is known to be Kluyveromyces
marxianus. The best optimation condition
for this yeast was as follows: concentration of carbon source (inulin) was
0.75%, pH was 5.5 and time of fermentation was 53 hours. In such condition K.
marxianus produced inulinase up to
0.6481 IU.
Penulis: Wijanarka, K Endang,
Hermin
Kode Jurnal: jpbiologidd090076
