TOXOPLASMA GONDII INFECTION ELICITS THE INCREASE OF FAS EXPRESSION ON THE TROPHOBLAST ASSOCIATED WITH THE INCREASE OF TROPHOBLAST APOPTOSIS
ABSTRACT: Toxoplasma gondii
infection causes the increase of trophoblast apoptosis. The mechanism of
trophoblast apoptosis in Toxoplasma gondii-infected pregnant mice has not been
fully understood. We measured Fas expression on the tropboblast of
infected-pregnant mice. The objective of this study was to proof the effect
Toxoplasma gondii infection to the increase of Fas expression on pregnant mice
trophoblast and the increase of Fas expression on pregnant mice trophoblast
assosiate with increase of trophoblast apoptosis. Forty-eight pregnant Swiss
mice were divided into 6 groups. Group I, first week gestation uninfected mice.
Group II, first week gestation infected mice. Group III, second week gestation
uninfected mice. Group IV, second week gestation infected mice. Group V, third week gestation uninfected
mice. Group VI, third week gestation
infected mice. Mice were infected with 1x103 tachizoites T. gondii doses by intraperitoneal. Four days
post infection, mice were sacrificed, placenta was removed for observation Fas expression
by immunohitochemistry staining and TUNEL ASSAY with Apop tag kit was used to
observe trophoblast apoptosis index. The data were analysed by ANOVA and
Regression. The result showed that Fas expression was significantly difference
(p < 0.00) between infected mice and uninfected mice trophoblast. Fas
expression was increasing on trophoblast of infected mice. Fas expression in first week gestation
infected mice increased 7.4 times (from 3.65% to become 27.11%); in second week
increased 7.2 times (from 5.37% to become 38.50%) and in the third week
increased 3.6 times (from 11.64% to become 41.44%). The increase of Fas
expression was significantly elevated when a high trophoblas apoptosis index in
infected mice, rather than uninfected mice. We concluded that Toxoplasma gondii
infection has effect on the increase of Fas expression on pregnant mice
trophoblast and Fas expression associated with the increase of trophoblast
apoptosis.
Author: Lucia Tri Suwanti,
Rochiman Sasmita, Suhartono Taat Putra
Journal Code: jpkedokterangg060010
