DNA ISOLATION FROM SWEAT STAIN IN CLOTHES AS FORENSIC IDENTIFICATION MATERIAL
ABSTRACT: DNA profiling is an
instrument to assist the investigation of either criminal or civil cases. It is
also highly useful in forensic identification. Until recently, personal
identification using sweat stain in clothes with DNA analysis method (DNA
profiling) has not been commonly used in Indonesia. A study has been conducted
on the sweat stain in clothes using DNA analysis for forensic identification
material. Loci in this study were CSF1PO, THO1, TPOX, vWA and D17S5. From 10
samples used, only 6 met the typing requirement (level >20ng/ml and purity
1- 2). As a comparison, we used blood from volunteers. Except the locus D17S5
which was not examined due to the small amount of DNA remains produced from
sweat stain, in electrophoresis visualization that presenting as bands, the
four loci in sweat and blood stain were identical/consistent. In conclusion,
sweat stain in clothes can be used as alternative material for forensic
identification using molecular forensic examination.
Author: Ahmad Yudianto, Soekry
Erfan Kusuma
Journal Code: jpkedokterangg060009
