Toxoplasma gondii Identification in Mother’s Blood and Fetal Tissue with Nested PCR
Abstrak: Toxoplasma gondii
identification in mother’s blood and fetal tissue with nested PCR Objective: To
examine the correlation between
Toxoplasma gondii infection with spontaneous abortion on pregnant women
based on nested PCR result from mother’s blood and fetal tissue. Methods: A prospective clinical diagnostic
study using nested PCR performed on 30 cases of pregnant women with spontaneous
abortion fulfilling the inclusion criteria, latex agglutination test (+) and
exclusion criteria, latex agglutination
test (-). Mother’s blood and fetal tissues samples, which gave positive
result in serologic test, were analyzed with nested PCR using18S-rDNA gene
primers. Results: Five of 30 mother’s blood samples (16.7%) and
9 of 30 fetal tissue samples (30%) gave positive PCR results. According to
Fisher’s Exact test, PCR detected the presence of Toxoplasma gondii in
significant value (P < .001). Conclusion: There is a strong correlation between
Toxoplasma gondii infection with spontaneous abortion (P < 001).
Author: Aloysius Suryawan,
Juliana A. Masengi-Rumopa, Josef S. B. Tuda
Journal Code: jpkedokterangg060005
