Implementation of 25-well culture plates for M. tuberculosis drug susceptibility testing in Indonesia
Abstract: At present, there is
no standardized method for Mycobacterium tuberculosis drug susceptibility
testing (DST) among laboratories in Indonesia. Since January 2001 to January
2004 we have tried to establish the method of 25-well culture plates with
middlebrook’s media (Drug Susceptibility Culture Plate (DSCP) method) used by
the Dutch Supranational Reference Laboratory at the Institute of Public Health
and the Environment (RIVM), Bilthoven, Netherlands. Our experience showed that
this method potentially gives better result as it can be very well
standardized, faster and provides detailed MIC (Minimal Inhibitory Concentration)
values. Data from 364 isolates that have been tested by DSCP method showed that
resistance to INH, rifampicin, ethambutol, and streptomycin were 21.4%, 19.8%,
15.7%, and 16.5% respectively. Multidrug resistance were found in 13.2%
isolates.
Author: Yeva Rosana, Tjahjani
M. Sudiro, Bachti Alisjahbana, Ida Parwati, Reinout van Crevel, Dick van
Soolingen
Journal Code: jpkedokterangg050126
