Cytosolic estrogen and progesterone receptor content in the endometriotic tissues and endometrium in women with and without endometriosis
Abstract: In order to have a
profile of cytosolic estrogen and progesterone receptors in either
endometriotic tissue or endometrium in patients with and without endometriosis
a cross-sectional study was performed involving 43 infertile women. They
consisted of 31 (72.09%) endometriosis and 12 (27.91%) non-endometriosis cases;
their average age was 32 ± 4 years and 32 ± 3 years respectively, with the
average length of menstrual cycle 31 ± 8 days and 29 ± 1 days respectively. The
endometriotic tissue was obtained by excision during operative laparoscopy
procedure, while the endometrium was obtained by biopsy following hysteroscopy
procedure. These procedures were conducted within the periovulatory period (on
Day 13-18 of the cycle). The sex steroid receptor content in the cytosol was
measured quantitatively using enzyme-immunoassay method, and calculated as sex
steroid receptor/cytosol protein (fmol/ml cytosol). It was found that the
average cytosolic estrogen receptor concentration in the respective tissues
were 512.99 fmol/ml in the endometriotic ovary compared with 2369.17 in normal
ovary and 632.18 fmol/ml in the endometriotic peritoneum compared with 9607.61
fmol/ml in normal peritoneum; while 99.28 fmol/ml and 608.33 fmol/ml in the
endometrium of women with endometriosis and those without endometriosis
respectively. The average cytosolic progesterone receptor concentration found
in the respective tissues were 50.64 fmol/ml in the endometriotic ovary
compared with 6469.42 fmol/ml in normal ovary and 1631.40 fmol/ml in
endometriotic peritoneum compared with 12466.99 in normal peritoneum, while
21.26 fmol/ml and 599.61fmol/ml in the endometrium of women with endometriosis
and those without endometriosis respectively. There is no significant
difference in the receptor concentration between each tissue according to its
topographic origin. However, this result may assume that the responsivity on
hormonal treatment in endometriosis cases will depend on the cytosolic sex
steroid receptor content in the sick tissues, and the peritoneal lesions will
possibly give better response than those in other sites. A further clinical
trial is necessary.
Author: T. Z. Jacoeb, C.
Nuruliza, N. Chaniago
Journal Code: jpkedokterangg050125
