Tingkat Kematangan Inti Oosit Sapi Setelah 24 Jam Presevasi Ovarium
ABSTRACT: The objective of the
research was to investigate their
meiotic competence or nuclear maturity of bovine oocytes maturity in vitro
after 24 hour preservation on 5°C. Oocytes
were collected by slicing the ovaries in modified phosphate buffer
saline (m-PBS). Selected cumulus-oocyte complexes (COCs) homogenous ooplasm were cultured in maturity medium at 38°C in
humidified atmosphere of 5% CO2 incubator. After 24 hours, oocytes stained for
nuclear maturity’s evaluation. The proportion of oocytes at metaphase II (MII)
was significantly difference (P< 0.05) on oocytes that 24 hours preservated (44.21 ± 3.04%) vs oocytes from fresh ovary
(73.97 ± 9.32%) (P<0.05). These results indicated that 24 hour’s
preservation bovine’s ovary on 50C cause
decreases of nuclear oocyte maturity.
Penulis: Rini Widyastuti, Siti
Darodjah Rasad
Kode Jurnal: jppeternakandd150068
