SEROLOGI DAN VIROLOGI VIRUS AVIAN INFLUENZA H5N1 PADA KUCING JALANAN DI KOTA BOGOR
ABSTRACT: Highly pathogenic avian influenza (HPAI) H5N1 virus
is a known pathogen in birds. Recently, the virus has been reported to cause
sporadic fatal disease in tigers, leopards, and other exotic felids as well as
domestic cats in Thailand. The present
study was carried out to investigate the presence of AI H5N1 virus infection in
stray cats roaming around residential, traditional and chicken farms in Bogor,
West Java. Ninety serum samples were tested using HI test to screened for the
presence of antibody to AI H5N1. Virus isolation was done in SPF embrionated
chicken eggs and identify using HI, AGP and RT-PCR. The results showed that
18,9% of stray cats developed antibodies against H5 with geometric mean titre
23,1 . Stray cats lived in traditional markets 18–40% developed antibodies in
the titre ranging from 22,8 to 24,5.
Only two out of nine stray cats which lived in chicken farm developed low
antibody titres again H5 (21). None of the stray cats lived in residencial area
have developed antibodies against H5. This study revealed that stray cats have
been contact with AI H5. Avian influenza H5 viruses were isolated in eight out
of 33 pooled of rectal swab samples. The viral cleavage site sequences are
CCTCAAAGAGAGAGC AGAAGAAAGAAGAGAGGT which represent amino acid sequences of
PQRESRRKKRG. Based on the cleavage site sequence, the isolates are similar with
the AI H5 virus subtype isolated from human in Indonesia during 2005–2007.
Penulis: Sri Murtini, R.
Susanti, Ekowati Handharyani
Kode Jurnal: jppertaniandd090081
