Peranan Fruktosa, Rafi nosa, dan Trehalosa pada Kriopreservasi Semen Kuda
ABSTRACT: The objective of
this study was to evaluate the effectiveness of carbohydrates supplementation
on the stallion semen cryopreservation. Semen was collected from 3 stallions
using artifi cial vagina twice a week. Collected semen was evaluated macro-and
microscopically. Semen showed >60% progressive motility was then divided
into 3 tubes and diluted with skim milk 1:1, centrifuged at 1006 g for 10
minutes. Supernatan was removed and each pellet rediluted either with skim milk
extender supplemented with 50 mM trehalose (ST); 50 mM raffi nose (SR) or 100 mM
fructose (SF) with the concentration of sperm were 200x106 ml-1. Extended semen packed into minitub 0.3 ml and
equilibrates at 4 oC for 2 hours, freezes in the liquid nitrogen vapor for 10
minutes and stored in liquid nitrogen container -196 oC for further evaluation. After 24 hours, the
semen was thawed at 37 oC for 30 second. The results of this experiment
indicated that trehalose supplementation in skim milk was found to signifi
cantly improve the percentage of sperm motility (P<0.05) for stallion 1 and
3 compared to raffi nose and fructose.
But in all stallions, trehalose and fructose were superior compared to raffi
nose.
Penulis: R. I. Arifi antini, B.
Purwantara, T. L. Yusuf, & D. Sajuthi
Kode Jurnal: jppeternakandd090121
