Kualitas Spermatozoa Tikus yang Diberi Tepung Kedelai Kaya Isoflavon, Seng (Zn) dan Vitamin E
Abstract: The objective of
this experiment was to evaluate the effects of isoflavone-riched soybean flour,
zinc (Zn) and vitamin E on quality of rats spermatozoa as animal model. Diet
was given as isonitrogen and isocaloric with 15% of dietary protein. Thirty
male Sprague Dawley post weaning rats (21 days old) were divided into six
groups and treated with isoflavone-riched soybean flour, Zn and vitamin E in
different combination. Isoflavone-riched soybean flour (3 mg/day) was given by
oral administration, whereas Zn and vitamin E were mixed with the basic diet.
The treatments were given for 2 months. Thirty female Sprague Dawley post
weaning rats (21 days old) were used to observe male fertility. Results
indicated that synergic interaction between isoflavone-riched soybean flour, Zn
and vitamin E increased motility and concentration of rats spermatozoa. The
treatment was not significant on relative weight of testis, conception rate and
foetus size. The best results was shown by the group which was given
isoflavone-riched soybean flour with diet containing both Zn and vitamin E,
i.e. motility rate of 79.50±1.12%, sperm concentration of 1636.90±87.95
million/ml, and abnormality rate of 9.00±0.70%. Conception rate and the number
of foetus on female rats were 100% and 11±0.71, respectively. It is concluded
that combination treatment of isoflavone-riched soybean flour, Zn and vitamin E
on male rats showed the best fertility in comparison with single treatment and
the other combination.
Penulis: S Astuti, D Muchtadi,
M Astawan, B Purwantara
Kode Jurnal: jppeternakandd090120
