KERAGAMAN DNA MIKROSATELIT BM415 DAN BM4621 PADA DOMBA KOMPOSIT (SUMATRA X BARBADOS BLACKBELLY X ST. CROIX)
Abstract: The forming
of composite breed
sheep were expected
to able adapted
with damp and
tropical environment, reproduction during
the year and
have good growth
rate. The genetic
variations of composite
sheep form to be analysed
in this research using microsatellite DNA marker. This research was to determine the allele variation of microsatellite
DNA in composite sheep by PCR (Polymerase Chain Reaction) using primers BM415
and BM4621 and the relation among litter size, litter weight at lambing and at
weaning, and genotype frequency in each primer. The PCR
products were visualized
on PAGE 8%
(Polyacrilamid Gel Electrophoresis) using
silver staining. The result
showed that composite
breed sheep population
exhibited the high
genotype variation, high
allele variation and high
heterozigosity. Loci BM415 showes 11 genotypes which identified 9 alleles, were
dominated EI (22,73%) and E (21,01%).
Loci BM4621 showes
11 genotypes which
identified 11 alleles,
DI and GK
genotypes and G allele
show high frequency
(17,05% and 20,45%
repectively). BM415 and
BM4621 loci showe
heterozigosity value of 0,851437
and 0,892905. The
average heterozigosity between
two locies were
0,872171. Heterozigosity and average heterozigosity value showed that the
composite sheep population in Stasiun Percobaan Cilebut, Balai Peternakan Bogor
has high variability.
Genotype was not
significantly different (P>0,05)
with litter size,
litter weight at lambing and at weaning.
Penulis: Ratna Kumala Dewi
Kode Jurnal: jppeternakandd100105
